Structural Changes in HPRT Gene of V79 Cells After Irradiation With Heavy Ions—Immediate and Delayed Effects

The radiobiological effects of accelerated ions with high charge and energy (HZE) on mammalian cells their propagation in time are still not sufficiently explained attract great deal attention. This work aims to compare the immediate delayed emphasis latter. As shown by our group, dependence mutant fraction expression after irradiation may have interesting, non-monotonic, character depending LET (linear transfer) used heavy ions. We speculate that this phenomenon occur due induced genomic instability. Another area research is study DNA structural changes these mutants at different times. Chinese hamster V79 were irradiated 11 B, 18 O, 20 Ne, gamma radiation. was ranging from 0.23 keV/?m 60 Co rays up 136 Ne unique HPRT isolated, concentration measured, exons amplified, analyzed several points, about 40 days, exposure. Over 1200 for deletions sorted into three main categories: partial deletion, PD—with deletion one eight exons; total deletions, TD—with all nine deleted; no deletions—no change structure observed. In general, number samples increasing radiation, suggesting higher deposition cell nucleus more likely cause larger changes. case increase observed ?115 followed a sharp decrease. also distribution particular various times, so-called mutational patterns. Hypothesis mechanisms behind phenomena given, possible implications further discussed.